This paper investigates the autographed library inventory of Sevillian canon Ambrosio José de la Cuesta y Saavedra (1653-1707), who was a careful collector of Spanish poetic compilations of the Golden Age and the author of the earliest additions to Nicolás Antonio’s Bibliotheca Hispana Nova. It offers a biographical sketch of Cuesta, a complete description of New York, Library of The Hispanic Society of America, MS. B2681, and a structural analysis of the works mentioned in the inventory contained in this manuscript. This essay also locates some of the 1287 works recorded in the same miscellaneous compilations, which are now preserved in the Colombina Library, the University Library of Seville and the Hispanic Society of America. Moreover, this article identifies several printed books by seventeenth-century authors from Seville which were owned by this bibliographer, offering a complete transcription and numbering of all the works contained in Cuesta’s inventory.